My Seq Tools: Primer Design Pipeline

Date stamp (record locator):

Please reload the form for each submission to generate a timestamp
or else your previous data will be overwritten.

Name of Primer Design Project: Tumour Xenograft Cell Mix Single Cell Misc

Enter the Sample ID eg (SA999):

Enter your list of hg19 SNV/SNP positions you want primers to be designed for
exactly as formatted below to ensure accurate automated processing.
chr1:23848591-23848591
chr1:48918790-48918790
chr1:75684963-75684963
Tested for up to 75 positions per submission - sample data
Tested using: Chrome, Safari but has issues on Firefox

Primer design for MiSeq run with:
2 x 150 bp PE (300 cycle run)

Enter primer3 conditions: Most stringent conditions (v0) Intermediate stringency (v0-1) Relaxed conditions (v0-2) Alternate conditions (250bp MiSeq run) (v1)

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